I mean come on, the plot is a banished princess fleeing to the woods and crossdressing as a man named Ganymede (which, you know, has some subtext-) to teach her crush how to effectively woo her. Orlando is bi and I WILL die on that hill, he literally pretends to marry "Rosalind" (aka Rosalind as Ganymede pretending to be Rosalind to teach Orlando how to woo her; to Orlando's knowledge, Ganymede is just a slightly androgynous cis man).
Phebe falls in love with Rosalind-as-Ganymede after being chastised by her as well (if I had a nickel for every time an otherwise ill-tempered woman in a Shakespearean comedy fell in love with a crossdressing woman after being scolded by her, I'd have two nickels. Which isn't a lot but it's weird that it's happened twice, the other being Olivia and Viola/Cesario in Twelfth Night); there's also a line at the end of the show where Hymen (goddess of marriage with an…unfortunate name that I had to say straight-faced since I played her) tells Phebe that she needs to marry Silvius or "have a woman to your lord", which in our current English is basically "marry Silvius or you might as well marry a woman, since no man would take you" Justice for Phebe tbh, I love the bitchy Shakespeare ladies sm!
A few people in my theatre group read Le Beau as being in love with Orlando (and I lowkey played it that way) because he goes back to warn him about the danger Duke Frederick poses when they've had no other onstage interaction, and he ends it with "hereafter, in a better world than this/I shall desire more love and knowledge of you"; there was some mild division over if Jaques was aro-ace, in love with Touchstone, or in love with Duke Senior and I basically toed the line between 1 and 3 and okay I'll stop rambling about the Shakespeare characters I get unhealthily attached to in every show I'm in-
A lot of people headcanon Rosalind as nonbinary but personally as far as Shakespearean Crossdressing Heroines Discourse goes I don't think Rosalind is nonbinary but from what I've read of Twelfth Night…I need to read the whole thing but enby Viola eyes emoji