The Soul Guardians, led by a smaller group known as the Soul Guardian Council (or just "the Council" for short), is a faction with the goal of ending The Creator's life and taking her powers. They are at constant war with the Hallowed Gladiator Organization. See Founding of the Soul Guardians for details on the group's origins.

The Soul Guardians enlist new members by spying on potential recruits to learn their weaknesses and insecurities, then taking advantage of these to convince the target that joining the group is the only way for them to be happy. The end goal is to recruit so many people and cause so much trouble that they draw the Creator's attention, forcing her into action to take back what was originally her idea.

The symbol of the Soul Guardians began as a circle with a jagged line drawn around it, but it gradually simplified to a five-pointed star. This makes it easier to quickly scrawl on walls without being seen, flagging safe spaces for the group.